Real-Time PCR System with Maximum Flexibility
Silent Features
  • 6 colors multiplexing capability
  • Excitation source is long live LEDs
  • Patented array of high performance optical fibers for loss-free excitation and detection of fluorescence
  • Highly sensitive Channel Photo Multiplier (CPM) detection system
  • Fast ramp rate for heating & cooling of 6.0ºC/sec and 4.5 ºC/sec respectively
  • Temperature gradient facility
  • Temperature range form 3-99ºC with temperature accuracy of 0.10º C
  • For maximum flexibility five different modules in various block formats ranging from 0.2 ml to 0.5ml tubes, upto 384 well formats are available
  • Performs absolute quantification, relative quantification, allelic discrimination and efficiency calculation
  • MIQE-compliant sample documentation
Modular block concept
The TOptical Thermocycler consists of the base unit of the TProfessional Thermocycler and the TOptical module. This modular system enables the upgrade of existing TProfessional Thermocyclers by the TOptical module to a complete Real- Time PCR Thermocycler. Simply insert the TOptical module and that’s it – in a few seconds you can begin with Real- Time PCR. Or the TOptical module can be quickly exchanged for a TProfessional module and the TOptical Thermocycler be used as conventional thermocycler. For maximum flexibility five different modules in various block formats, ranging from 0.5 ml tubes up to 384 well microplates, are available.

Optional gradient function
Finding the best annealing temperature is, especially for Real-Time PCR experiments very important. Often time consuming optimisation tests are conducted only limited, which leads to suboptimal results under routine conditions. To allow the testing of new primer combinations in a single run, the TOptical module optionally is available with gradient function. With a gradient span of 40 °C and the linear gradient tool for programming of equal temperature increments between the 12 columns of the block the TOptical fulfills every wish for easy optimisation of Real-Time PCR assays. New primer pairs with unknown annealing temperatures can be tested quickly and new protocols are optimized in a very short time.

Flexible configuration with optical filter modules
The patented optical system of the TOptical Thermocycler provides a filter wheel for 6 color filter modules. The filter wheel can be freely equipped with color filter modules of choice. Furthermore, the system can also subsequently be upgraded with color filter modules and so the range of applications extended. In total 10 different color filter modules are available, covering the wh ole range of commonly used Real-Time PCR dyes, from the blue to the red absorption spectrum, including filter modules specially optimized for FRET applications.

LED technology
The fluorescent dyes are excited by three LEDs in the colors blue, white and red. The combination of these three LEDs allows optimal excitation of fluorescent dyes over a wide spectral range. Unlike with so-called „wide blue“ LEDs especially the short-wavelength blue spectral range and the long-wavelength red range are optimally covered. In combination with the high-performance optical fibers and the highly sensitive Channel Photo Multiplier intensity losses in the quantum efficiency are avoided to ensure maximum sensitivity. Due to the excitation of each single well the use of passive reference dyes is not necessary, allowing multiplexing of up to six targets. The longevity of the LEDs makes a regular exchange of the light source superfluous and thus helps avoid recurring costs.

Software
The TOptical Thermocycler is controlled by PC software qPCRsoft. The software is especially developed to provide ease of use and clear arrangement of functional elements. It includes the same easy spreadsheet or graphical programming function as the TProfessional Thermocycler. Information on sample is entered in a well arranged plate scheme that provides a comprehensive overview at a glance. Typical evaluation methods of the Real-Time PCR such as ΔΔCt method, absolute quantification, relative quantification, allelic discrimination and efficiency calculation are already integrated. In addition, export tools for further external analysis programs like Excel, REST, qBASE and Biogazelle are available and the software provides MIQE-compliant documentation of experiments.