Pacific biosciences has introduced a platform for single molecule, real time detection of biological events designed to transform the future of medicine, agriculture and biofuels.

Single molecule, real-time (SMRTTM) detection provides direct measurement of individual molecules, capturing multiple dimensions of data. Templates can be prepared for the PacBio RS without PCR amplification, resulting in more uniform sequence coverage across genomic regions regardless of GC content, facilitating the detection of minor variants in heterogeneous samples. While observing single molecule sequencing by a highly possessive strand displacing DNA polymerase in real time, the system also records the kinetics of each nucleotide incorporation reaction, identifying base modifications of the native templates, such as DNA methylation.

Single Molecule Real-Time Analysis Single molecule, real-time (SMRT™) detection provides direct measurement of individual molecules, capturing multiple dimensions of data. Templates can be prepared for the PacBio RS without PCR amplification, resulting in more uniform sequence coverage across genomic regions regardless of GC content, facilitating the detection of minor variants in heterogeneous samples. While observing single molecule sequencing by a highly processive strand-displacing DNA polymerase in real time, the system also records the kinetics of each nucleotide incorporation reaction, identifying base modifications of the native templates, such as DNA methylation

Flexibilit Customize your experiments with granular, low cost consumables & adjustable run conditions. Multiple sequencing protocols provide maximum flexibility for a wide range of applications.

Longer Readlength With readlengths in the thousands of bases, the PacBio RS can resolve both SNPs and large-scale structural rearrangements. Long reads increase understanding of disease heritability through haplotype phasing. Long reads also simplify and improve genomic assembly by reducing the number of contigs, & produce superior consensus accuracy compared to other technologies at the same depth of coverage.

Faster Time to Resul Read DNA at the speed of the polymerase in bases per second. With rapid sample preparation completed in 4-6 hours & short run times measured in minutes, you can obtain biologically meaningful answers within hours instead of a week or more. For data analysis, we offer a robust suite of SMRT Analysis tools to speed up your time from data to insights.

Ease of Us Prepare samples in a few hours without amplification. Monitor sequencing in real time, with feedback on the state of the PacBio RS, its contents, and your experiment through a touch-screen interface. Analyze data and view results in rich user interfaces built around industry standards. The entire workflow was designed with the user in mind – from sample preparation, to instrument use, to data analysis